Fig. 8: IL-1α-induced neuroinflammation and oligodendrocyte loss is partly mediated by astrocytic IL-1R1. | Nature Communications

Fig. 8: IL-1α-induced neuroinflammation and oligodendrocyte loss is partly mediated by astrocytic IL-1R1.

From: The alarmin interleukin-1α triggers secondary degeneration through reactive astrocytes and endothelium after spinal cord injury

Fig. 8

a Genetic design of the GfapCre::Il1r1r/r mouse line. b Quantification of Il1r1 gene expression in primary brain astrocytes from adult Il1r1r/r mice expressing an IL-1R1-knockout phenotype (set to 0%) and GfapCre::Il1r1r/r mice (n = 6 mice/group). c Quantification of Ly6G+ neutrophils that infiltrated the spinal cord of WT, Il1r1r/r and GfapCre::Il1r1r/r mice at 24 h post-i.c.m. injection of PBS or rmIL-1α (n = 7 WT + PBS, n = 8 Il1r1r/r + PBS, n = 5 GfapCre::Il1r1r/r + PBS, n = 7 WT + rmIL-1α, n = 8 Il1r1r/r + rmIL-1α, n = 6 GfapCre::Il1r1r/r + rmIL-1α). d Quantification of Olig2+ CC1+ mature oligodendrocytes in the spinal cord at 24 h post-injection (n = 8 WT + PBS, n = 8 Il1r1r/r + PBS, n = 7 GfapCre::Il1r1r/r + PBS, n = 8 WT + rmIL-1α, n = 8 Il1r1r/r + rmIL-1α, n = 6 GfapCre::Il1r1r/r + rmIL-1α). e, f Locomotor function was assessed using the BMS score (e) and BMS subscore (f) after SCI (n = 16 WT, n = 15 Il1r1r/r, n = 15 GfapCre::Il1r1r/r). g Genetic design of the GfapCre::Il1r1fl/fl mouse line. h Il1r1 gene expression in primary brain astrocytes isolated from adult Il1r1fl/fl mice, which normally express the Il1r1 gene (set to 100%), and GfapCre::Il1r1fl/fl mice (n = 5 mice/group). i Quantification of spinal cord-infiltrated neutrophils at 24 h post-injection (n = 8 WT + PBS, n = 8 Il1r1fl/fl + PBS, n = 8 GfapCre::Il1r1fl/fl + PBS, n = 8 WT + rmIL-1α, n = 7 Il1r1fl/fl + rmIL-1α, n = 7 GfapCre::Il1r1fl/fl + rmIL-1α). j Quantification of mature OLs at 24 h post-injection (n = 13 WT + PBS, n = 8 Il1r1fl/fl + PBS, n = 6 GfapCre::Il1r1fl/fl + PBS, n = 9 WT + rmIL-1α, n = 6 Il1r1fl/fl + rmIL-1α, n = 7 GfapCre::Il1r1fl/fl + rmIL-1α). k, l BMS scores (k) and subscores (l) after SCI (n = 13 WT, n = 16 Il1r1fl/fl, n = 13 GfapCre::Il1r1fl/fl). All data are means + /− SEM and statistical significance was determined by a two-tailed Student’s t-test (b, h), two-way ANOVA (c, d, i, j), or two-way repeated measures ANOVA (e, f, k, l) followed by Bonferroni post-hoc test. e, f ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05; &&&&p < 0.0001, &&&p < 0.001, &&p < 0.01, &p < 0.05. Other pairwise comparisons and p-values are indicated in graphs.

Back to article page