Fig. 3: TFEB stimulation of MAT1A transcription.
a Male 10 weeks old C57BL/6 J mice were intravenously injected with Ad-Null or Ad-TFEB at 5 × 108 pfu/mouse. One week later, mice were fed chow (C) or Western diet (WD) for one additional week. Relative liver mRNA are shown with control arbitrarily set as 1. (n = 5). MAT1A Methionine adenosyltransferase 1A, CBS cystathionine β-synthase, CSE cystathionine γ lyase. b, c Male 10 weeks old C57BL/6 J mice were intravenously injected with Ad-shCon (Ad-scramble) or Ad-shTFEB at 1 X 109 pfu/mouse. Mice were fed chow for 2 weeks and euthanized after 6 h fast (n = 8). Liver protein (b) and mRNA (c) were measured. Each band represents an individual mouse sample in (b). d Luciferase reporter constructs containing 1.9 kb or 0.9 kb human MAT1A promoter and TFEB expression plasmids were transfected in triplicates in AML12 cells for 48 h. Luciferase activity was normalized to β-galactosidase activity. A representative of 2 independent experiments. e, f Chromatin immunoprecipitation assay with human liver nuclear fraction (e) or male 10 weeks old C57BL/6 J mouse liver nuclear fraction (f). NC negative control with IgG, IP immunoprecipitation with anti-TFEB antibody. Primer pair locations are relative to translational start site as +1. Results in a and c are expressed as mean ± SEM. Results in (d, e, and f) are expressed as mean ± SD of technical triplicate repeats. Two-way ANOVA and Tukey post hoc test are used for a and unpaired 2-tailed Student’s t-test is used for (c, d, e, f). A p value < 0.05 is considered statistically significant. Source data for (a–f) are provided as a Source Data file.
