Fig. 4: TFEB stimulates hepatic CoA synthesis to support fatty acid metabolism. | Nature Communications

Fig. 4: TFEB stimulates hepatic CoA synthesis to support fatty acid metabolism.

From: TFEB regulates sulfur amino acid and coenzyme A metabolism to support hepatic metabolic adaptation and redox homeostasis

Fig. 4

a Illustration of the Coenzyme A (CoA) synthesis pathway. PANK: patothenate kinase. b, c Male 10 weeks old C57BL/6 J mice were intravenously injected with Ad-Null or Ad-TFEB at 5 × 108 pfu/mouse. One week later, mice were fed chow (C) or Western diet (WD) for one additional week. Mice were fasted for 6 h and euthanized. Relative abundance of liver metabolites are shown with control arbitrarily set as 1 (n = 4). d, e, f Adenovirus injected male 10 weeks old C57BL/6 J mice (Ad-Null and Ad-TFEB at 5 x 108 pfu/mouse and Ad-shCon (Ad-scramble) and Ad-shPANK1 at 1 x 109 pfu/mouse) were fed WD for 2 weeks and euthanized after 6 h fast (n = 5). d Representative liver Oil Red O stain. Scale bar = 250 μm. e Liver triglycerides (TG). f Liver cholesterol (Chol). Results in (b, c, e, f) are presented as mean ± SEM. Detailed statistical analysis of (b) and (c) is described under Metabolomics, statistical and bioinformatics analysis in the Methods section, and 2-way ANOVA and Tukey post hoc test are used for e and f. A p value < 0.05 is considered statistically significant. Source data for (b, c, e, f) are provided as a Source Data file.

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