Fig. 4: FLP-3 and INS-5 signalling peptides control UPS-dependent protein turnover. | Nature Communications

Fig. 4: FLP-3 and INS-5 signalling peptides control UPS-dependent protein turnover.

From: Thermosensation in Caenorhabditis elegans is linked to ubiquitin-dependent protein turnover via insulin and calcineurin signalling

Fig. 4

a Pathways (Reactome) significantly (q-value < 0.01) affected in dyf-1(mn335) compared with WT identified by GSEA (Methods), based on microarray data. Each pathway is represented by a bubble, size of a bubble: size of the pathway (number of its gene members affected). Thickness of edges reflects proportion of genes shared between 2 pathways. Colour of nodes reflects average change of the pathway gene members, red: the genes are upregulated, blue: genes are down-regulated. b Heatmap of differentially expressed neuropeptides (q-value < 0.01) in dyf-1(mn335) compared with WT based on microarray analysis. Colour of cells: log2 fold change of gene expression in dyf-1(mn335) compared with WT, n = 4 biologically independent experiments. c Worms expressing the UFD substrate and the mutations indicated were grown and analysed as in (Fig. 2b). d Quantification of UbV-GFP levels of worms from (c) and additionally dyf-1(mn335); ins-1(tm1888). 6 (dyf-1(mn335), dyf-1(mn335); flp-3(ok3265), dyf-1(mn335); ins-5(tm2560)) and 3 (dyf-1(mn335); ins-1(tm1888), dyf-1(mn335); ins-5(tm2560); flp-3(ok3265)) biologically independent experiments. dyf-1(mn335); flp-3(ok3265) p = 0.0002, dyf-1(mn335); ins-1(tm1888) p = 0.312, dyf-1(mn335); ins-5(tm2560) p < 0.0001, dyf-1(mn335); ins-5(tm2560); flp-3(ok3265) p < 0.0001 compared with dyf-1(mn335), dyf-1(mn335); flp-3(ok3265) versus dyf-1(mn335); ins-5(tm2560); flp-3(ok3265) p = 0.1994, dyf-1(mn335); ins-5(tm2560) versus dyf-1(mn335); ins-5(tm2560); flp-3(ok3265) p = 0.2797. Column graph: mean ± standard deviation, scatter plots: individual experiments, same colour: same experiment ****p ≤ 0.0001, ***p ≤ 0.01, n.s. p > 0.05. One-way ANOVA followed by Dunnett’s multiple comparisons test. e, f Indicated mutant worms expressing the UFD substrate were analysed as in (c). Typical result of n = 3 (e) and 4 (f) biologically independent experiments. Additional quantifications see Supplementary Fig. 5, Source data are provided as a Source Data file.

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