Fig. 6: dyf-1(mn335) mutant exhibits impaired thermotaxis behaviour.

a A simplified model of thermotaxis neural circuit. Temperature is sensed by the thermosensory neuron AFD. The AIY-RIA and AIZ-RIA circuit promotes the movement of animals to a higher temperature (thermophilic behaviour) and lower (cryophilic behaviour) temperature than the cultivation temperature, respectively. The RIA interneuron integrates the thermal signals and drives thermotaxis behaviour. b The linear thermal gradient setup established on a 12*12 cm agar surface. Tstart indicates the temperature at which the animals were placed on the linear gradient after they were cultivated at 22 °C. c Negative thermotaxis behaviour of animals cultivated at 22 °C after 60 min are shown. The percentage distribution of animals in the specified temperature ranges are indicated. Number of animals are WT 552, dyf-1(mn335) 1117, gcy-8(oy44) 759, dyf-1(mn335); gcy-8(oy44) 810 and ttx-3(ks5) 815. d Linear thermal gradient setup similar to (b), but worms were cultivated at 15 °C, and positioned at Tstart of 19 °C. e Negative thermotaxis behaviour of animals cultivated at 15 °C. T start = 19 °C. Number of animals are WT 569, dyf-1(mn335) 691, gcy-8(oy44) 497, dyf-1(mn335); gcy-8(oy44) 510 and ttx-3(ks5) 581. For detailed statistics see Supplementary Tables 2 and 3. 3 biologically independent experiments (c, e). No gradient is displayed in Supplementary Fig. 7a–d. f Negative thermotaxis behaviour of animals cultivated at 22 °C after 60 min are shown. Number of animals are WT 654, dyf-1(mn335) 831, str-2(tm445); odr-7(tm4791) 1209, gcy-8(oy44); str-2(tm445); odr-7(tm4791) 720, and ttx-3(ks5) 978. 3 biologically independent experiments. For detailed statistics see Supplementary Table 4. Source Data are provided as a Source Data file.