Fig. 7: Sensory temperature perception adjusts protein turnover. | Nature Communications

Fig. 7: Sensory temperature perception adjusts protein turnover.

From: Thermosensation in Caenorhabditis elegans is linked to ubiquitin-dependent protein turnover via insulin and calcineurin signalling

Fig. 7

a Fluorescence and Nomarski images of worms expressing the UFD substrate (UbV-GFP) in WT or dyf-1(mn335) mutant worms grown at different temperatures as indicated. DiI denotes ciliated amphid and phasmid neurons. n = 3 biologically independent experiments. Scale bar 100 μm. b Protein lysates of worms displayed in (a) were analysed by SDS-PAGE and immunoblotting against GFP and tubulin as loading control. n = 3 biologically independent experiments. c Lysates of WT or dyf-1(mn335), gcy-8(oy44) or dyf-1(mn335); gcy-8(oy44) mutant worms grown at indicated temperatures were analysed as in (b) n = 3 biologically independent experiments. Long exp. = long exposure of the blot. Source Data are provided as a Source Data file.

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