Fig. 6: Differential association of TRDV2-containing CDR3 sequences with effector modules and with public CDR3γ sequences in the human fetal thymus.

γδ CDR3 region was amplified from cDNA of fetal thymic sc RNA-seq libraries (n = 6). CDR3 data and transcriptomes were matched via cellular barcodes. A Plots indicate distribution (UMAP; left) and percentage (paired dot plots; center and right) of cells containing a CACDTGGYWDTRQMFF or CACDTGGYSWDTRQMFF TRDV2 sequence. B Plots indicate distribution (UMAP; left) and percentage (paired dot plots; center and right) of cells containing a CACDWGSSWDTRQMFF or CACDYWGSSWDTRQMFF TRDV2 sequence. Light gray dots in the UMAPS indicate cells with CDR3δ sequences different than the highlighted ones or cells without detected CDR3δ sequence. C Cells containing different public TRDV2 sequences were split in two groups based on their CDR3γ pairing: cells paired with the public CALWEVQELGKKIKVF TRGV9-TRGJP chain (gray color) and cells paired with the public CATWDTTGWFKIF TRGV8-TRGJP1 chain (red color). The percentages were calculated based the total number of cells containing a TRDV2 chain paired with TRGV9-TRGJP or TRGV8-TRGJP1 gamma chains, respectively. I-M vs EFF comparison in A, B was analyzed with two-tailed paired t-test, while comparisons between effector clusters were performed by Ordinary one-way ANOVA for matched data with Holm-Sidak’s multiple comparisons test as Post Hoc test when data followed normality or Friedman test followed by Dunn’s test as Post Hoc test when data did not follow normality. Data in C was analyzed by two-tailed paired t-test. Error bars in A, B, C correspond to the SEM. “I-M” group: immature/maturing. “EFF” group: effector. “T1”, ”T3”, and “T2” groups: Type 1, Type 3, and Type 2-like. Source data are provided as a Source data file. See also Supplementary Fig. 9.