Fig. 8: Downregulation of IP6 biosynthesis disrupts Integrator function.
From: Inositol hexakisphosphate is required for Integrator function
a Schematic of the U7snRNA reporter systems, where the U7 gene is cloned upstream of GFP or luciferase. Top: in WT cells, the ICM drives RNA Pol II termination and prevents GFP/luc expression. Bottom: Upon losing ICM function through IPMK depletion, RNA Pol II productively elongates through the GFP/luc ORF, yielding expression. The Integrator cleavage site is indicated with the red arrow. b Western blot analysis of 293 T cell lysates treated with siRNA as labeled and the U7-GFP reporter. The levels of IntS11 and IPMK depletion are shown, and the level of GFP expression from the reporter. c Similar experiment as in panel b except the U7-luc reporter was used, and light units were measured in each case. Results are shown from biologically independent replicates, depicting averages and standard deviations (mean ± SD, n = 3). Source data are provided as a Source Data file.
