Table 1 Cryo-EM data collection, structure refinement, and validation statistics
From: Inositol hexakisphosphate is required for Integrator function
Drosophila IntS4-IntS9-IntS11 complex (ICM) | |
|---|---|
Data collection and processing | |
Magnification | 81,000 |
Voltage (kV) | 300 |
Electron exposure (e–/Å2) | 51 |
Defocus range (μm) | −1 to −2.5 |
Pixel size (Å) | 1.083 |
Symmetry imposed | C1 |
Image stacks (no.) | 3083 |
Initial particles images (no.) | 2,920,144 |
Final particle images (no.) | 620,438 |
Map resolution (Å) | 2.74 |
FSC threshold | 0.143 |
Map sharpening B-factor (Å2) | −81.8 |
Refinement | |
Number of protein residues | 1621 |
Number of metal ions | 2 |
Number of atoms | 12,819 |
R.m.s. deviations | |
Bond lengths (Å) | 0.010 |
Bond angles (°) | 1.093 |
PDB validation | |
Clash score | 6.34 |
Poor rotamers (%) | 0.07 |
Ramachandran plot | |
Favored (%) | 90.11 |
Allowed (%) | 9.89 |
Disallowed (%) | 0.00 |
