Fig. 5: Immobility-related oscillatory activity in the ferret is abolished by atropine.
20 s of example data from ferret F4 in a control session (a) where no atropine was administered (No-drug control, −A) and test session (b) with atropine administered (+A). Top: Head speed showing multiple periods of immobility and movement. Middle: LFP trace (1 Hz high-pass filtered, 50 Hz notch filtered) from a channel estimated to be below the cell layer. Bottom: LFP spectrogram, normalised to maximum power. Examples of autocorrelation peak range during (c) immobility (speed < 5 cms−1) and (d) locomotion (speed > 20 cms−1), without (−A; immobile: grey, moving: orange) and with (+A; green) administration of atropine. Top: 1 s example LFPs. The mean head speed in the example epochs are indicated. Bottom: Corresponding autocorrelograms of the LFP epochs above (black line) overlaid on the matched sinusoid wave autocorrelograms (grey line). Black vertical line indicates the uncorrected peak range measurement for these examples, vertical grey line indicates peak range of matched sinusoid used for normalisation. e Comparison of autocorrelation peak range for data during locomotion without (−A; grey) and with (+A, green) atropine for 3 channels across 2 ferrets. Each marker represents a 1 s data epoch (F1R −A n = 862, +A n = 1203; F4periCL −A n = 972, +A n = 1250; F4subCL −A n = 2077, +A n = 3133). Box plot centres show median, box bounds show interquartile range and whiskers show 9th to 91st percentile. f β1 coefficients (markers) for linear mixed-effects models predicting peak range values based on drug condition for immobile and moving data independently. Error bars show 5–95% confidence intervals. *p < 0.001 (LMM). g as in e but for moving data.
