Fig. 3: ∆glgX mutant accumulates increased levels of glycogen.
From: Allosteric regulation of glycogen breakdown by the second messenger cyclic di-GMP
a Transmission electron micrographs of colonies stained for α-glucans of S. venezuelae WT, ∆glgX and a strain overexpressing glgX from the constitutive ermE* promoter. Strains were grown for 5 days on MYM agar at 30 °C. b The glycogen content of 5 days-old colonies of S. venezuelae WT, ∆glgX, ∆glgX complemented with FLAG-tagged glgX expressed from the native promoter and ∆glgX overexpressing glgX from the ermE* promoter was quantified using the glycogen assay kit (MAK016) from Sigma-Aldrich. Data are presented as a mean of three biological repeats +/- SD. c Macrocolonies of S. venezuelae WT, ∆glgX, and WT either containing the empty pIJ10257 vector or expressing glgX from the strong ermE* promoter. Strains were grown on MYM-agar containing 0.03% maltose for 48 h.
