Fig. 3: Desynchronized glutamate release events reveal quantal size.
From: Vesicular release probability sets the strength of individual Schaffer collateral synapses
a iGluSnFR changes in fluorescence (single bouton) in response to single APs in ACSF containing 4 mM [Ca2+]e (blue traces). Red arrow indicates stimulation onset. Synaptic release probability was 1 (no failures). The black arrowhead indicates the start of 4 mM [Sr2+]e wash-in. During wash-in of 4 mM [Sr2+]e discrete events appear in the wake of the stimulation (green arrowheads). Scale bars: 20 ms, 2 ΔF/F0. b Amplitude histogram of evoked responses measured in 4 mM [Ca2+]e (blue bars) and delayed events measured in 4 mM [Sr2+]e (green bars) and fluctuation of baseline fluorescence (gray bars) of the example shown in a. c Second example. Baseline fluctuations are plotted with narrow bin size to show their normal distribution. Source data are provided as a Source Data file.
