Fig. 4: Introducing the active Dadh variant into the non-dopamine metabolizing strain E. lenta DSM 2243 results in dopamine dehydroxylation.

a Schematic of pXD70DA7, pXD70DAmt1, and pXD70DA9 constructs. b LC-MS/MS to quantify the production of dopamine dehydroxylation metabolite m-tyramine after incubation with corresponding E. lenta cultures for 48 h. Data represented as mean ± SD with n = 4 biological replicates. Source data are provided as a Source Data file.