Fig. 1: Zfp335 is critical to αβ T-cell development.
From: Loss of Zfp335 triggers cGAS/STING-dependent apoptosis of post-β selection thymocytes
a Gating schema for ex vivo analysis thymocyte development beginning with live thymocytes (DAPI− CD90.2+, Fig S11a). b Total thymic cellularity in WT (Cre-negative) or Zfp335fl/fl E8III-cre (Zfp335cKO) mice. Total numbers (c) and frequency (d) of TCRγδ+ cells in WT or Zfp335cKO thymuses. Numbers (e) and frequencies (f) of DN, DP, and SP thymocyte subsets in WT or Zfp335cKO thymi. Numbers (g) and frequencies (h) of early DN1-DN4 thymocyte subsets in WT or Zfp335cKO thymuses. i Relative cells numbers in DN3-SP thymocyte subsets represented as percent of WT mean. j Volcano plot of differentially expressed genes between Zfp335cKO and WT by RNA-seq. k Overlap between Zfp335 ChIP-seq (GSE58293) and differentially expressed genes in Zfp335cKO and WT DP. l Gene Set Enrichment Analysis of differentially expressed genes (k). Positive enrichment scores indicate pathways positively enriched in Zfp335cKO cells. a–i Cre-negative WT (n = 11) and Zfp335cKO (n = 12) male and female mice from four independent experiments. P-values determined by two-tailed Mann–Whitney U-test (b–d) or two-way ANOVA with post hoc Sidak’s test (e–i). j–l RNA-seq analysis of Zfp335+/+ E8III-cre or Zfp335cKO DP thymocytes (n = 3 each) of 6-week-old female mice from one experiment. Plots show mean ± SEM. Data are compiled from one (j–l) or 5 independent experiments (a–i). Source data are provided as a Source Data file.
