Fig. 4: Defining the ‘true’ DN4 thymocyte population at the single-cell level.
From: Loss of Zfp335 triggers cGAS/STING-dependent apoptosis of post-β selection thymocytes
a UMAP projection and identification of 10 clusters identified in full scRNA-seq dataset. b UMAP colored by cell cycle phase. Blue or green identify actively cycling cells. c Frequency distributions for WT (n = 6357) and Zfp335cKO (n = 5392) cells across the ten clusters. d Dot plot of key cell-type-defining genes. e Violin plots of positive selection signature genes in thymocytes51. f Representative gating for CD4 vs. CD8 expression on day 3 of OP9-DL1 cultures seeded with WT Thy1.1 retrovirus transduced (EV+) DN3 or DN4 cells or non-transduced (EV−) DN4 cells. g Representative TCRβ expression among DN, DP, CD4SP, or CD8SP cells from f. Numbers indicate geometric MFI of TCRβ expression. a–e Data are from one experiment. f, g Data representative of two independent experiments. Source data are provided as a Source Data file.
