Table 2 Forward and reverse primers used for qRT-PCR

From: Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids

Gene

Forward primer (5′-3′)

Reverse primer (5′-3′)

DAPL1

CTCGGAAAGGGGGACATCCT

AGTTGAGCTTCTCCAGTGCG

GAPDH

CTCTCTGCTCCTCCTGTTCGA

TGAGCGATGTGGCTCGGCT

GATA3

GCCCCTCATTAAGCCCAAG

TTGTGGTGGTCTGACAGTTCG

HAVCR1

GTTCCTCCAATGCCTTTGCC

CGGTGTCATTCCCATCTGTTG

HNF4A

ACCCTCGTCGACATGGACA

GCCTTCTGATGGGGACGTG

HOXD11

GCCAGTGTGCTGTCGTTCCC

CTTCCTACAGACCCCGCCGT

LHX1

CGTCATTCAGGTCTGGTTCC

CCCGTAGTACTCGCTCTGGT

PARAXIS

GGGGGTGGCCGTCGT

CAGGCTGAATGGATCCTCAC

SIX1

AAAGGGAAGGAGAACAAGGATAG

GGAGCCTACATGATTACTGGG

SIX2

TCCTGGTCCCTCCGTATGTA

TAGGGGCAGATAGACCACCA

TMEM-100

CAGGCGTTGCTGTTTCTTGT

CAGGGTGAAAGCTCGGAGAG

WNT4

AACTGCTCCACACTCGACTC

TGACCACTGGAAGCCCTGT

  1. Gene names are italicised.
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