Fig. 2: HGD induces global transcriptomic changes in young but not aged animals. | Nature Communications

Fig. 2: HGD induces global transcriptomic changes in young but not aged animals.

From: The unfolded protein response reverses the effects of glucose on lifespan in chemically-sterilized C. elegans

Fig. 2

a Experimental design of bulk RNA-seq. A single pool of synchronised worms was grown on ND until day 1 of adulthood (D1). From the same single pool, a subset of worms was fed on HGD at day 1 (HGD-1). The second subset of worms grown on ND was fed on HGD at day 5 (HGD-5) of adulthood (D5). Wrms fed ND, HGD-1, and HGD-5 were harvested at D8 (n = 6). b Venn diagram represents differentially expressed genes in HGD-1 and HGD-5 compared to ND. c Hierarchical clustering of the top 10,000 differently expressed genes in all samples. d Volcano plots of HGD-1 and HGD-5 compared to ND where coloured data points represent differentially expressed genes. Functional annotation analysis in HGD-1 (e) and HGD-5 (f).

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