Fig. 3: Mutation of PT led to the formation of fruit with pointed tips.
From: Variation in the fruit development gene POINTED TIP regulates protuberance of tomato fruit tip
a Fruit phenotypes of plants harboring PTR and PTH alleles from the F2:3 population. b Fruit phenotypes of PTH overexpressing lines and wild-type (TS-9, PTH allele). c Morphology of the distal end of fruit from the PTH overexpressing lines and wild-type (TS-9). d Fruit phenotypes of PTH overexpressing lines and wild-type (TS-3, PTR allele). e Schematic illustration of two sgRNA target sites in PT. The red lines indicate sgRNAs. f–h Genomic DNA sequences of PTH mutants (f: CR-ptH-1, CR-ptH-4, and CR-ptH-10), PTR mutants (g: CR-ptR-3, CR-ptR-5, and CR-ptR-6; h: CR-ptR-1, CR-ptR-4, and CR-ptR-7) and the pertinent wild-type sequences. The black dotted lines indicate the base sequences between sgRNAs. TS-9 harbors the PTH allele and produces non-pointed tip fruit. TS-3 and LA4053 harbor the PTR allele and produce fruit with pointed tips. i–k Phenotype of fruit from the CR-ptH mutants (i: CR-ptH-1, CR-ptH-4, and CR-ptH-10), CR-ptR mutants (j: CR-ptR-3, CR-ptR-5, and CR-ptR-6; k: CR-ptR-1, CR-ptR-4, and CR-ptR-7) and the pertinent wild-type. l Phenotype of fruit from the CR-ptH/PTRpro:PTR lines and the pertinent control CR-ptH.
