Table 1 Distribution of alleles in single cell-derived clones edited at MTOR by co-selection

From: Marker-free co-selection for successive rounds of prime editing in human cells

MTOR mutation

% of clones with indicated alleles

WT

WT + large insertion(s)

Monoallelic PE*

Biallelic

Triallelic

PE*

PE&

E2419K

0% (0/30)

0% (0/30)

40% (12/30)

33% (10/30)

27% (8/30)

F2108L

0% (0/31)

6% (2/31)

26% (8/31)

19% (6/31)

48% (15/31)

I2017T

4% (1/27)

11% (3/27)

37% (10/27)

22% (6/27)

26% (7/27)

Total

1% (1/88)

6% (5/88)

34% (30/88)

25% (22/88)

34% (30/88)

  1. Compilation of genotypes from Supplementary Fig. 14.
  2. *Except for two MTOR-F2108L clones, all remaining alleles harbor small indels, large insertions and deletions, or pegRNA scaffold incorporation (See Supplementary Fig. 14).
  3. &Homozygous, only PE-specified sequences are detected.
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