Table 1 Kinetics and affinity constants for wild-type and mutant PldA–PA3488 complex

From: Structural insights into PA3488-mediated inactivation of Pseudomonas aeruginosa PldA

Variants

Association rate Ka (1/Ms)

Dissociation rate Kd (1/s)

Binding affinity KD (M)

PldA

5.96E + 05

1.83E−05

3.07E−11

PldAD643A

2.17E + 04

1.83E−05

8.46E−10

PldAR820A

2.13E + 03

5.19E−08

2.44E−11

PldAR969A

1.18E + 04

7.57E−05

6.42E−09

PldAD643A-R820A-R969A

2.40E + 03

1.78E−06

7.41E−10

PA3488R50A

2.08E + 05

2.14E−04

1.03E−09

PA3488D118A

3.30E + 05

6.25E−06

1.90E−11

PA3488D273A

6.92E + 03

1.20E−04

1.73E−08

PA3488R50A-D118A-D273A

2.71E + 05

3.82E−03

1.41E−08

  1. Source data are provided in Supplementary Fig. 10.
Back to article page