Fig. 6: MAPK cascades regulate the in vivo phosphorylation level of FTZ-F1. | Nature Communications

Fig. 6: MAPK cascades regulate the in vivo phosphorylation level of FTZ-F1.

From: A single transcription factor facilitates an insect host combating Bacillus thuringiensis infection while maintaining fitness

Fig. 6

a–c Effect of silencing MAP4K4 gene on FTZ-F1 mRNA expression (a), protein level and degree of phosphorylation (b), and larval mortality (c) in the resistant strain NIL-R. d–f Effect of specific inhibitors of p38, ERK, or JNK on FTZ-F1 mRNA expression (d), protein level and degree of phosphorylation (e), and larval mortality (f) in the resistant strain NIL-R. Relative mRNA expression levels (a, d) were quantitated and normalized to the expression level of the RPL32 gene, the value for the buffer treated strain was set as 1. Phosphorylated and non-phosphorylated FTZ-F1 proteins (b, e) were separated on a Phos-tag SDS-PAGE gel, detected by anti-FTZ-F1 and quantitated by densitometry using the ImageJ 1.51 software and normalized to the β-actin. Data were presented as mean values ± SEM (a, c, d, f). n = 3 biologically independent samples, ns, not significant, p values are shown. The differences between control and dsRNA-treated groups or inhibitor-treated groups were tested by one-way ANOVA with Tukey’s test. Source data are provided as a Source Data file.

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