Fig. 7: CRISPR/Cas9-mediated knockout of non-receptor genes in P. xylostella. | Nature Communications

Fig. 7: CRISPR/Cas9-mediated knockout of non-receptor genes in P. xylostella.

From: A single transcription factor facilitates an insect host combating Bacillus thuringiensis infection while maintaining fitness

Fig. 7

a Representative chromatogram of CRISPR/Cas9-induced mutation of ABCC1 gene (top), double-gene knockout of APN6 and APN5 genes (middle), and triple mutant of ABCC1/APN6/APN5 genes by introducing mutations on the base of the double-mutant (bottom). The cleavage site of Cas9 protein is indicated with a red-edged yellow triangle. The arrow indicates the junction position after deletion. b Representative pupal morphology in P. xylostella susceptible DBM1Ac-S, resistant NIL-R, and non-receptor gene-edited strains. c–f Evaluation of fitness costs in non-receptor gene mutant strains. A series of biological parameters were compared among non-receptor gene knockout strains (C1KO, N6-5KO, and C1/N6/N5KO) with the susceptible DBM1Ac-S and the parental NIL-R resistant strains. c Pupation rate. d Pupal weight. e Pupal duration. f Hatching rate. Data were presented as mean value ± SEM, n = 5 biologically independent samples with ten larvae per replicate, ns, not significant, p values are shown. One-way ANOVA with Tukey’s test was used for comparison. Source data are provided as a Source Data file.

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