Fig. 3: TGF-β plus IL-4 signaling increases DBP but decreases E2F8 during Th9 differentiation. | Nature Communications

Fig. 3: TGF-β plus IL-4 signaling increases DBP but decreases E2F8 during Th9 differentiation.

From: Opposing functions of circadian protein DBP and atypical E2F family E2F8 in anti-tumor Th9 cell differentiation

Fig. 3

a RT-PCR analysis and b Western blotting of Stat5, DBP and E2F8 in WT and Tgfbr1 KO CD4+ T cells cultured with TGF-β, IL-4 or TGF-β plus IL-4. Expression of Stat5a, DBP and E2F8 during TGF-β and IL-4 stimulation in WT, S213A- or EPSM-Smad3 mutants transfected CD4+ T cells c, d or p38 inhibitor-pretreated CD4+ T cells e, f as in a, b. a, c, e The data were representative of four independent experiments or b, d, f three experiments. Data were analyzed by two-way ANOVA with Tukey’s test. Graphs show the mean ± SEM. Source data are provided as a Source Data file.

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