Fig. 4: Increased DBP but decreased E2F8 binding at Il9 gene during Th9 differentiation. | Nature Communications

Fig. 4: Increased DBP but decreased E2F8 binding at Il9 gene during Th9 differentiation.

From: Opposing functions of circadian protein DBP and atypical E2F family E2F8 in anti-tumor Th9 cell differentiation

Fig. 4

a Identification of Stat5a, DBP and E2F8 binding elements in the Il9 gene. b–d ChIP assay analysis for binding of Stat5a, DBP and E2F8 to the promoter region of the Il9 during Th9 differentiation. e, g Expression of Il9 mRNA and f, h ELISA of IL-9 and in CD4+ T cells transfected with Stat5a, Dbp or E2f8 specific shRNA e, f or overexpressing virus g, h followed by stimulation with TGF-β plus IL-4. O/E, Overexpressed. b–d, f These data were representative of three independent experiments or e, g, h four experiments. These data were analyzed by b, d two-tailed unpaired Student’s t test or e–h one-way ANOVA with Tukey’s test. Graphs show the mean ± SEM. Source data are provided as a Source Data file.

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