Fig. 5: The functions of DBP and E2F8 in human TH9 differentiation. | Nature Communications

Fig. 5: The functions of DBP and E2F8 in human TH9 differentiation.

From: Opposing functions of circadian protein DBP and atypical E2F family E2F8 in anti-tumor Th9 cell differentiation

Fig. 5

a RT-PCR analysis and b Western blotting of STAT5, DBP and E2F8 in human CD4+ T cells cultured with TGF-β plus IL-4 for 5 days. c Expression of Il9 mRNA after 3 days and d ELISA of IL-9 after 5 days in human CD4+ T cells transfected with STAT5A, DBP or E2F8 specific siRNA, followed by stimulation with TGF-β plus IL-4. e Western blotting of STAT5, DBP and E2F8 and f IL-9 protein were measured by ELISA in human CD4+ T cells cultured with TGF-β plus IL-4 with or without p38 inhibitor for 5 days. a, c, f These data are representative of four independent experiments or b, d, e three experiments. These data were analyzed by a two-tailed unpaired Student’s t-test or c, d, f one-way ANOVA with Tukey’s test. Graphs show the mean ± SEM. Source data are provided as a Source Data file.

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