Fig. 2: In-depth characterization of DNAJB1-PRKACA-specific CD8+ T cells and single-cell TCR sequencing. | Nature Communications

Fig. 2: In-depth characterization of DNAJB1-PRKACA-specific CD8+ T cells and single-cell TCR sequencing.

From: The oncogenic fusion protein DNAJB1-PRKACA can be specifically targeted by peptide-based immunotherapy in fibrolamellar hepatocellular carcinoma

Fig. 2

a Heat map of single-cell RNA sequencing analysis of flow cytometry-based bulk sorted PA*24-specific CD8+ T cells of a healthy volunteer (HV) 1 and HV2 after aAPC-based priming with HLA-A*24-PA*24-monomer, showing log normalized gene expression for selected activated T cell markers. b Specific cell lysis by PA*24-specific CD8+ T cells from HV2, of PA*24-loaded autologous CD8- target cells (gray fill, dashed line (upper panel); red line (lower panel)) at various effector-to-target cell ratios compared to negative peptide-loaded autologous CD8- target cells (white fill, solid line (upper panel)). PA*24-unspecific CD8+ T cells showed no lysis of the target cells (black line (lower panel). Results are shown for three independent technical replicates. c Flow cytometry-based bulk sort of PA*24-specific CD8+ T cells of two HVs (HV1, HV2) after aAPC-based priming with HLA-A*24-PA*24-monomer (left panel) for single-cell T cell receptor (TCR) sequencing. The right panel depicts physiochemical properties and amino acid sequences of the CDR3-α/-β region of the most frequent TCR clone from each donor in comparison to their target peptide PA*24. On the y-axes, the hydrophilicity according to the Hopp-Woods scale82 is indicated and amino acids (AA) are grouped by their physiochemical properties with color code. Source data are provided as a Source Data file.

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