Fig. 10: Stem-like CD8+ T cells differentiate into TRM-like cells in TDLN of human head and neck cancer patients.
From: TGF-β-dependent lymphoid tissue residency of stem-like T cells limits response to tumor vaccine
a The association of CD69+CD103+% in total CD8+ TEM isolated from proximal TDLN (left) and tumor (right) are shown (n = 24 individual patients). Simple linear regression was performed. b The percentage of CD69+CD103+ cells in total CD8+ TEM isolated from proximal TDLN, distal LN and tumor from the same patients (n = 28 patients with three samples from each patient). c Top, representative FACS profiles of total CD8+ T cells isolated from non-metastatic TDLN, metastatic LN and tumor are shown; bottom, compared with TCF-1+PD-1−CD45RA+ naive CD8+ T cells, FACS profiles of pre-gated TCF-1+PD-1+ stem-like CD8 T cells to show the expression of CD69 and CD103. d % CD69+CD103+ cells in pre-gated stem-like CD8+ T cells are shown (dLN, n = 10, meta LN, n = 8 and tumor, n = 6). Blue, proximal dLN; black, distal LN; red, tumor; and red circle filled with blue, metastatic dLN. Data are presented as mean ± SEM. Each symbol represents the results from an individual patient. ****p < 0.0001 in b are calculated by paired Student t-test and indicated p values in d are calculated by Ordinary one-way ANOVA with multi-comparison posttest. Two-sided tests were used. Source data are provided as a Source data file.
