Fig. 6: Tcf-1+ Pmel-1 T cells in TDLN are antigen-experienced T cells and differentiate into Tcf-1- effectors in response to tumor vaccine.
From: TGF-β-dependent lymphoid tissue residency of stem-like T cells limits response to tumor vaccine
a Experimental design. b–d In the absence of tumor vaccine. e–g Side-by-side comparison of unvaccinated vs d7–8 post vaccine. b Representative histograms of pre-gated Tcf-1+ Pmel-1 T cells isolated from TDLN, total TIL Pmel-1 T cells and host-derived bulk naive CD8 are shown. In b, black line, WT and red line, Tgfbr2−/−; solid line, TDLN Tcf-1+Pmel-1; dotted line, TIL Pmel-1; and gray shade, bulk TDLN CD8. c Normalized MFI of Slamf6, PD-1 and CD38 as well as the percentage of CD44hi and CD62L+ cells in Tcf-1+ TDLN Pmel-1 T cells and TIL Pmel-1 T cells are shown. For Slamf6, PD-1 and CD38, bulk LN CD8, n = 7 and other groups, n = 5. For bulk endogenous CD8 and LN stem-like Pmel-1 T cell comparison, tumor samples were excluded. For CD44 and CD62L, n = 3/each. In c, gray filled circle, bulk LN CD8; black, WT; red, Tgfbr2−/−; filled, TDLN Tcf-1+ Pmel-1; and empty, TIL Pmel-1. d Representative FACS profiles of TDLN Pmel-1 T cells overlaid with TIL Pmel-1 T cells isolated from the same animal. e The percentage of Tim3+Tcf-1− cells in Pmel-1 T cells are shown (No Vaccine groups, n = 9/each and Vaccine groups, n = 12/each). In d and e, red, TDLN; blue, TIL; filled, WT; and empty, Tgfbr2−/−. f Representative FACS profiles of TDLN Pmel-1 T cells are shown. g The percentage of Granzyme A+Tcf-1− (left, no Vaccine groups, n = 12, WT + Vaccine, n = 11, and Tgfbr2−/−+Vaccine, n = 13), IFN-γ+ (middle), and IFN-γ+TNF+ (right) in TDLN Pmel-1 T cells are shown (No Vaccine groups, n = 7/each and Vaccine groups, n = 6/each). Black, WT and red, Tgfbr2−/−. Each symbol in c, e, and (g) represents the results from an individual mouse. 2–3 independent repeats. Data are presented as mean ± SEM. N.S., not significant (p > 0.05), ****p < 0.0001 and indicated p values are calculated by Ordinary one-way ANOVA with Tukey’s multi-comparison posttest. Two-sided tests were used. Source data are provided as a Source data file.
