Fig. 3: Alternative splicing of LRRFIP2 regulates metastasis-associated gene network in gastric cancer cells.

a Heatmap showing the downregulated genes in exon 7 of LRRFIP2 variant 3-deleted MKN1 cells. Threshold values are as follows: corrected value P < 0.01 and absolute log2-fold change >1.0. b KEGG pathways and GO terms enriched in differentially expressed genes downregulated by exon 7 deletion from a. c Real-time qRT-PCR showing the expression of altered target genes in exon 7-deleted MKN1 cells. d Real-time qRT-PCR showing the expression of the altered target genes from c in MKN28 cells stably expressing LRRFIP2 variant 2 and 3. e Box plots showing SERPINE1 expression levels in each tumor stage in gastric cancer tissues from public TCGA data sets. T1-T4 refers to the size and or/extent of the main tumor into nearby tissues. The center line is the median; the box is from the 25th to the 75th percentile. The upper or lower whisker extends from the hinge to the 1.5 x IQR (distance between the first and third quartiles) from the hinge for up and low, respectively. f Kaplan-Meier analysis showing relapse-free survival depending on SERPINE1 expression levels from public meta-analysis data (N = 875). P values for c, d were calculated by unpaired two-tailed Student’s t tests. P values for e were calculated by two-sided Wilcoxon rank sum tests. P value for f was calculated from log-rank test. c, d Data are representative mean ± SD of three independent samples (N = 3). n.s: not significant. Source data are provided in the Source Data file.