Fig. 3: Bulk RNA-seq analysis of individual CARMz T (sCARMz T), CARMz T from a mixture of CARMz T and CARP T (mCARMz T) and CARMz T cells treated with anti-PD-L1 mAb (CARMz T + AZ) after coculture with HeLa-GL cells.

a–d Bulk RNA-seq strategy: individual CARMz T (sCARMz T), CARMz T from a mixture of CARMz T and CARP T (mCARMz T) and CARMz T cells treated with AZ (20 μg/ml) (CARMz T + AZ) were isolated by flow cytometry sorting based on their GFP tag post-coculture with HeLa-GL cells, and then performed bulk RNA-seq analysis (N = 3 biological samples). The red dots represent AZ. b The numbers of genes upregulated and downregulated in CARMz T + AZ and mCARMz T compared with sCARMz T. c The heatmap shows clustering of differential expressed genes (DEGs) in sCARMz T, mCARMz T, and CARMz T + AZ (N = 3 biological samples). Cutoff: absolute log2 (fold change) ≥ 1; adjusted P value ≤0.05. d GSEA illustrating the enrichment of genes in WNT signaling pathway in mCARMz T versus sCARMz T. NES (Normalized Enrichment Scores) and normalized p-values are indicated. e The production of IL5, IL10, and IL13 by CARP T, CARMz T, a combination of CARMz T and CARP T and control CAR19z T cells post-coculture with HeLa-GL cells. Data are presented as mean ± SD (N = 3 independent experiments). p Values were calculated by two-way ANOVA with Tukey’s multiple comparisons test (CARMz T + CARP T vs. CARMz T = IL5: 1.016E−07, IL10: 0.009, IL13: 7.235E−07). f Percentage of IL13-scecreting cells in individual CARMz T (sCARMz T), CARMz T from a mixture of CARMz T and CARP T (mCARMz T) and CARMz T cells treated with AZ (20 μg/ml) (CARMz T + AZ) post-coculture with HeLa-GL cells at a 1:1 E:T ratio for 24 h (gated on CD3⁺GFP⁺ cells). *p < 0.05, **p < 0.01 and ***p < 0.001.