Fig. 1: Lats1/2 deficiency leads to fat remodeling.
From: The Hippo pathway links adipocyte plasticity to adipose tissue fibrosis
a–d Male WT or ob/ob mice were analyzed at 12 weeks old. a, Real-time quantitative PCR (RT-qPCR) analysis of genes involved in Hippo pathway and fibrotic response in scWAT (n = 5 mice). b, Immunoblot analysis of LATS1/2, YAP/TAZ, YAP phosphorylated at Ser112 (p-YAP) and TAZ phosphorylated at Ser89 (p-TAZ) in scWAT. c Quantification of protein expression from scWAT shown in b (n = 4 mice). d Quantification of phosphorylation levels of YAP/TAZ (n = 4 mice). e–h Male WT mice were fed a HFD or ND for 18 weeks. e RT-qPCR analysis of genes involved in Hippo pathway and fibrotic response in scWAT (n = 5 mice). f Immunoblot analysis of LATS1/2, YAP/TAZ, p-YAP and p-TAZ in scWAT. g Quantification of protein expression from scWAT shown in f (n = 3 mice). h Quantification of phosphorylation levels of YAP/TAZ (n = 3 mice). i–m Male Lats1f/fLats2f/f (L1L2-FF) and Lats1f/fLats2f/f AdipoqCre (L1L2-AKO) mice were analyzed at 5 weeks old. i Quantification of body fat mass by DXA (n = 6 mice). j Quantification of scWAT, vWAT and BAT weight (n = 10 mice). k Representative sections of scWAT with H&E staining or Masson’s trichrome staining at the indicated age. l Immunoblot analysis of adipocyte identity and fibrotic protein expression. m RT-qPCR for adipocyte and fibrotic marker gene expression in scWAT (n = 6 mice). Data are means ± SEM. Two-tailed unpaired Student’s t test; *P < 0.05, **P < 0.01, ***P < 0.001. Exact P values are provided in a Source data file.
