Fig. 3: Lats1/2 deficiency induces AT fibrosis in obese mice.
From: The Hippo pathway links adipocyte plasticity to adipose tissue fibrosis
a mRNA expression of Tgfb1/2/3 in scWAT of male mice fed a ND or HFD for 18 weeks from 6 weeks old (n = 5 mice). b Immunoblot analysis of phosphorylation of SMAD2/3 (p-SMAD2/3) of mice fed a ND or HFD for 18 weeks. Right, quantification of protein and relative phosphorylation levels of SMAD2/3 (n = 3 mice). c mRNA expression of Tgfb1/2/3 in scWAT of 12-week-old male WT or ob/ob mice (n = 5 mice). d Immunoblot analysis of protein expression of p-SMAD2/3 of mice in 12-week-old male WT or ob/ob mice. Right, quantification of protein and relative phosphorylation levels of SMAD2/3 (n = 4 mice). e, f Eight-week-old male Lats1f/fLats2f/f mice were injected with AAV-ADP-GFP or AAV-ADP-Cre in scWAT for 4 weeks. e Immunoblot analysis of LATS1 or LATS2 knockout efficiency in scWAT. f mRNA expression of fibrotic and adipocyte markers in scWAT (n = 6 mice). g Experimental outline: male L1L2-FF mice were fed a HFD for 16 weeks from 6 weeks old, followed by a local injection with AAV-ADP-GFP or AAV-ADP-Cre in scWAT, and all were analyzed after 4 weeks. h RT-qPCR analysis of fibrosis markers in scWAT of mice in g (n = 5 mice). i RT-qPCR analysis of fibrosis markers in scWAT of 12-week-old male Lats1f/fLats2f/fob/ob mice injected with AAV-ADP-GFP or AAV-ADP-Cre (n = 5 mice). j, k Representative scWAT sections with Masson’s trichrome staining (j) or Picrosirius red staining (k) of mice in i. Data are means ± SEM. Two-tailed unpaired Student’s t test; *P < 0.05, **P < 0.01, ***P < 0.001. Exact P values are provided in a Source data file.
