Fig. 4: Lats1/2 deficiency in adipocytes elicits a CCL2/CCL7-macrophage feedforward loop that increases TGFβ expression in macrophages.

a Expression of F4/80, Col1a1, and Acta2 of L1L2-FF (n = 6) and L1L2-AKO (n = 5 at P1, n = 6 at P7, P14 and P21) mice during growth. b Representative scWAT sections stained for F4/80 of 3-week-old male L1L2-FF or L1L2-AKO mice. Independent experiments were performed three times with similar results. c Quantification of F4/80⁺ cell percentages in scWAT SVF of 3-week-old male L1L2-FF or L1L2-AKO mice (n = 6). d, e Quantification of CD206⁺CD11c⁻ (d) and CD206⁻CD11c⁺ (e) cell percentages in scWAT SVF of 3-week-old male L1L2-FF or L1L2-AKO mice (n = 6). f mRNA expression of inflammatory markers in scWAT of 3-week-old male L1L2-FF or L1L2-AKO mice (n = 6). g mRNA expression of inflammatory genes in male L1L2-FF and L1L2-iAKO mice that were locally injected with AAV-CAG-GFP (n = 5) or AAV-CAG-TGFβR1 (T204D) (n = 6) in scWAT. h mRNA expression of inflammatory genes in male L1L2-FF or L1L2-iAKO mice that were locally injected with AAV-CAG-TGFβ1 (2CS) in scWAT (n = 5). i mRNA expression of inflammatory genes in male L1L2-FF or L1L2-iAKO mice that were locally injected with AAV-EF1A-DIO-TGFβR1 (T204D) in scWAT (n = 4). j Eight-week-old male L1L2-iAKO mice were subcutaneously injected with AAV-CAG-GFP (n = 7) or AAV-CAG-CCL2/CCL7 (n = 5) and analyzed for mRNA expression of inflammatory genes and fibrosis markers 4 weeks later. k F4/80⁻ and F4/80⁺ cells were magnetically sorted from pooled WT scWAT SVF and analyzed for mRNA expression of Tgfb1/2/3 (n = 3). scWAT from three male mice were pooled as one sample. l BMDMs were treated with Veh, and IL4 plus IL13 for 24 h and analyzed for Mrc1, Ccl2 and Ccl7 mRNA expression (n = 4 biologically independent cell cultures). Data are means ± SEM. Two-tailed unpaired Student’s t test in a, c–f, h–l; two-way ANOVA with Bonferroni’s multiple-comparisons test in g; *P < 0.05, **P < 0.01, ***P < 0.001. Exact P values are provided in a Source data file.