Fig. 5: Lats1/2 deficiency promotes cell fate conversion from adipocytes to myofibroblasts.

a Immunoblot analysis of protein expression of GFP in SVF and adipocyte from 8-week-old Adipoq^CreRosa26^mTmG scWAT. Independent experiments were performed twice with similar results. b Flow cytometry analysis of percentages of live CD45⁻CD31⁻GFP⁺DPP4⁺ cells in scWAT SVF of P21 L1L2-FF^{LSL-CAS9-EGFP} or L1L2-AKO^{LSL-CAS9-EGFP} reporter mice (n = 5). c Representative sections of scWAT of P14 or P28 L1L2-AKO^{LSL-CAS9-EGFP} mice stained for αSMA (red), DPP4 (purple), nucleus (DAPI, blue) and GFP (green). Arrowheads indicate examples of GFP⁺DPP4⁺ cells. Arrows point to GFP⁺αSMA⁺ cells. Independent experiments were performed three times with similar results. d Sorted cell subsets from P21 L1L2-AKO^mTmG scWAT SVF were plated and stained for αSMA (AF647, red) and nucleus (DAPI, blue). e Quantification of percentages of GFP+DPP4+ (n = 6), GFP+DPP4- (n = 7), GFP-DPP4+ (n = 7), GFP-DPP4- (n = 7) cells. Dots represent cell percentages of random visual fields from P21 L1L2-AKOmTmG mice. Independent experiments were performed twice with similar results. f Sorted GFP⁺DPP4⁺ cells from P21 L1L2-AKO^{LSL-CAS9-EGFP} scWAT SVF were treated with TGF-β1 (10 ng/ml) for 0 h or 24 h. Cells were stained with αSMA (red), DPP4 (purple), nucleus (DAPI, blue) and GFP (green). An arrowhead points to the GFP⁺αSMA⁻DPP4⁺ cell. Independent experiments were performed twice with similar results. g Sorted GFP⁺DPP4⁺ cells from L1L2-AKO^{LSL-CAS9-EGFP} reporter scWAT SVF were analyzed for DPP4 expression in GFP⁺ cells before or after transplantation into scWAT of 8-day-old L1L2-AKO recipient mice. One representative transplant from n = 3 biological replicates. Data are means ± SEM. One-way ANOVA with Bonferroni’s multiple-comparisons test in e; two-tailed unpaired Student’s t test in b. *P < 0.05, **P < 0.01, ***P < 0.001. Exact P values are provided in a Source data file.