Fig. 3: Interrelationship between CANX, CALR, and PDIA3 in EBOV-GP1,2 (GP) downregulation. | Nature Communications

Fig. 3: Interrelationship between CANX, CALR, and PDIA3 in EBOV-GP1,2 (GP) downregulation.

From: RNF185 regulates proteostasis in Ebolavirus infection by crosstalk between the calnexin cycle, ERAD, and reticulophagy

Fig. 3

A Expression of PDIA3, CALR, and CANX was determined in HEK293T WT, single PDIA3-, CALR-, or CANX-KO, and dual PDIA3/CALR- or PDIA3/CANX-KO cells. Protein expression was determined by WB. PDIA3, CALR, and CANX were detected by their specific antibodies. B GP was expressed with indicated ER proteins in HEK293T WT and indicated KO cells. Protein expression was detected by WB. GP was detected by anti-EBOV-GP; PDIA3 and CALR were detected by anti-Myc; HSPA5 was detected by anti-FLAG; CANX was detected by anti-HA. C GP was expressed with PDIA3, CALR, or CANX in HEK293T cells. Cells were treated with indicated amounts of Tizoxanide (TIZ), and protein expression was determined by WB. GP was detected by anti-EBOV-GP; PDIA3 and CALR were detected by anti-Myc; CANX was detected by anti-HA.

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