Fig. 2: Chemical synthesis of cyclic peptide analogue of 1 and their affinity screening for Lys63-linked Di-Ub.
From: Selective macrocyclic peptide modulators of Lys63-linked ubiquitin chains disrupt DNA damage repair
a General presentation for screening of the cyclic peptide library, chemically prepared to employ Fmoc-SPPS. b Preparation of cyclic peptide 1 (CP1). c CP1 with a Cys residue at various positions in the peptide sequence. d The binding affinity of cyclic peptides to Lys63-linked Di-Ub, normalized to the affinity of CP1. Data were plotted as mean ± SD for n = 3 biologically independent experiments and each in triplicates. e Concentration dependent binding study for FITC-labeled cyclic peptide 1 (CP1-FITC). Mean binding response values for each concentration of CP1-FITC were taken for n = 3 biologically independent experiments (black, red, and green) and the binding curve (black line) determining the KD (95.8 ± 2.3 nM) of CP1-FITC. All source data are provided as a Source data file.
