Fig. 3: The binding affinity of cyclic peptides 1 and 2 against Ub chains with different linkages and lengths.
From: Selective macrocyclic peptide modulators of Lys63-linked ubiquitin chains disrupt DNA damage repair
a Binding of cyclic peptide 1 on Lys11 linked Di-Ub, which indicated no binding by SPR. (Red: original trace, Black: fitting curve). b Binding of cyclic peptide 1 on Lys29-linked Di-Ub, which indicated no binding by SPR. (Red: original trace, Black: fitting curve). c Relative binding of cyclic peptides 1 and 2 on linear Di-Ub. Data were plotted as mean ± SD for n = 3 biologically independent experiments. d Relative binding of cyclic peptides 1 and 2 on Lys6-linked Di-Ub. Data were plotted as mean ± SD for n = 3 biologically independent experiments. e Relative binding of cyclic peptides 1 and 2 on Lys48-linked Di-Ub. Data were plotted as mean ± SD for n = 3 biologically independent experiments. f Relative binding of cyclic peptides 1 and 2 on Lys48-linked Tetra-Ub. Data were plotted as mean ± SD for n = 3 biologically independent experiments. All source data are provided as a Source data file.
