Fig. 5: Identifying interactors of peptide 31.
From: Selective macrocyclic peptide modulators of Lys63-linked ubiquitin chains disrupt DNA damage repair
a Schematic workflow of sample preparation and analysis by proteomics using biotin-conjugated cyclic peptides. b Ub chains were pulled down with peptides 31 and scrambled control (38) and detected by antibodies for Lys63 and Lys48-linked Ub chains in western blot analysis. Representative of n = 3 biologically independent samples. c Volcano plot (using Perseus 1.6.7.0.) showing the differentially enriched proteins. Proteins involved in processes mediated by Lys63-linked Ub are highlighted as; protein transport: blue, DNA repair: red, histone modification: green, and cell cycle: purple. A paired t-test was performed for n = 3 parallel samples. d Gene ontology (GO) analysis of genes from (c) with at least threefold enrichment compared to scramble control 38 (two-tailed, unpaired t-test, and p-values were given in the plot). All source data are provided as a Source data file.
