Fig. 5: In vivo real-time quantitative analysis of PpIX metabolism and sO2 levels by FL/PA duplex imaging.
From: Synthetic biology-instructed transdermal microneedle patch for traceable photodynamic therapy
a In vivo PpIX FL images of A375 tumor-bearing mice visualized using an IVIS spectrum system after viaious treatments (I: Control; II: MN-CCP; III: MN-CCPC; IV: MN-CCPA; V: MN-CCPCA). b The FL intensity of the tumor site after administration of MN patches at the indicated time points. Data represent the means ± SD from three biologically independent female A375 tumor-bearing mice, n = 3. c Biodistributions of different MN patch-derived PpIX in skin and tumor were monitored by FL cross sections (red from PpIX and blue from Hoechst), scale bar = 200 μm. Images are from three biologically independent tumors of 4-week-old female A375 tumor-bearing mice, n = 3. d Representative PA images of sO2 in A375 tumors before and after viaious treatments at a given time interval. All the groups were irradiated with a 635 nm laser (200 mW cm−2, 10 min) after testing the sO2 at the 24 h time point. e Quantitative analysis of the sO2 in the total tumor area at different time points. Data represent the means ± SD from three biologically independent female A375 tumor-bearing mice, n = 3. Source data are provided as a Source data file.
