Fig. 8: Enhanced therapeutic efficacy and biosafety of MN-CCPCA patch for U87MG tumors.
From: Synthetic biology-instructed transdermal microneedle patch for traceable photodynamic therapy
a In vivo PpIX FL images of U87MG tumor-bearing mice after administrations of 5-ALA i.v., 5-ALA i.t., MN-5-ALA, CCPCA i.v., CCPCA i.t., and MN-CCPCA patch at the indicated time points. b PpIX FL intensities of tumor tissues after different treatments at the indicated time points. Data represent the means ± SD from three biologically independent female U87MG tumor-bearing mice, n = 3. c Colocalization FL images of intratumoral PpIX by cross sections after different treatments at 12 h post-treatment (red FL signal from PpIX and blue FL signal from Hoechst), scale bar = 200 μm. Data represent the means ± SD from three biologically independent female U87MG tumor-bearing mice, n = 3. d Bioluminescence images of U87MG tumor-bearing mice (n = 5) treated with 5-ALA i.v., 5-ALA i.t., MN-5-ALA, CCPCA i.t., MN-CCPCA (S), MN-CCPCA (M) on days 0 and 14. The symbols “S” indicate single laser irradiation and “M” indicate multiple laser irradiation. e Relative tumor volume growth curves, f tumor weight, and g body weight in different treatment groups on day 14. Data represent the means ± SD from five biologically independent female U87MG tumor-bearing mice, n = 5. Statistical significance was calculated with two-tailed Student’s t test. h In vivo immunofluorescence images of anti-GSH (green) and Hoechst (blue) in 4T1 tumors after different treatments, scale bar = 1 mm. Images are from five biologically independent tumors of 6-week-old female BALB/c-nude mice, n = 5. i HIF-1α-, H&E-, and TUNEL-stained sections of U87MG tumors after various treatments indicated, scale bar = 100 μm. Images are from five biologically independent tumors of 6-week-old female BALB/c-nude mice, n = 5. j Serum biochemical analysis of U87MG tumor-bearing mice in CCPCA i.t. and MN-CCPCA groups before and after 30 min laser irradiation. Data represent the means ± SD from three biologically independent blood samples of 6-week-old female U87MG tumor-bearing mice, n = 3. Statistical significance was calculated with two-tailed Student’s t test. Source data are provided as a Source data file.
