Fig. 5: Adipose tissue inflammation in Ai-α4KO mice. | Nature Communications

Fig. 5: Adipose tissue inflammation in Ai-α4KO mice.

From: Phosphatase protector alpha4 (α4) is involved in adipocyte maintenance and mitochondrial homeostasis through regulation of insulin signaling

Fig. 5

a Top upregulated KEGG pathways between Control and Ai-α4KO in BAT (left) and iWAT (right). b Heatmap of genes involved in the cytokine-cytokine receptor interaction, with the color intensities indicating the Z-score of each sample. Expression levels of inflammatory cytokines, chemokines (c), and macrophage (d) markers in BAT from Control and Ai-α4KO mice on day 9 (n = 12/group). (Two-tailed Student’s t-test, **p < 0.01; ***p < 0.001). e Staining of iWAT and BAT sections from Control and Ai-α4KO mice on day 9 for F4/80. Scale bars =50 μm. f Flow cytometric analysis results showing infiltrated immunocytes with CD11c and CD206 on day 9. Representative data corresponding to iWAT are indicated as the FACS profiles (left). The ratio of M1 (CD11c+) to M2 (CD11c+) macrophages infiltrated in iWAT and BAT are shown in the right graph (n = 8/group). (Two-tailed Student’s t-test, *p = 0.03, ***p < 0.0001). g TUNEL staining results corresponding to iWAT and BAT sections from Control and Ai-α4KO mice on day 9. Scale bars = 50 μm. The arrow shows TUNEL-positive cells. h Immune-stained iWAT and BAT sections from Control and Ai-α4KO mice for the identification of cleaved caspase-3 on day 9. Scale bars = 50 μm. The arrow shows cleaved caspase-3 cells. i Expression levels of genes related to NF-κB signaling pathways and inflammasome components measured via real-time qPCR using BAT from Control and Ai-α4KO mice on day 9 (n = 12/group). Data are presented as mean ± SEM (Two-tailed Student’s t-test, ***p < 0.0001). Source data are provided as a Source Data file.

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