Fig. 4: G protein–binding interface mediated by the ILC2 of CRF2R. | Nature Communications

Fig. 4: G protein–binding interface mediated by the ILC2 of CRF2R.

From: Structure insights into selective coupling of G protein subtypes by a class B G protein-coupled receptor

Fig. 4

a Comparison of ICL2 conformations in the UCN1-CRF2R-G11, UCN1-CRF2R-Go and UCN1-CRF2R-Gs structures (PDB: 6PB1). The red arrows indicate the relative orientation differences of the different Gα. b Sequence alignment of αN and β1 in the different Gα proteins. c Interactions between ICL2 and Gs. d Interactions between ICL2 and G11. The side chains of receptor residues, and N198, I199, and K345 of G11 in this panel were truncated in the structure. Those residues shown here were prepared based on the Rosetta-refined model. e Interactions between ICL2 and Go. The polar contacts are shown as purple dashed lines. f G protein activation and signaling assays of wild-type (WT) and ICL2 mutant CRF2R using a Gαs-Gβγ dissociation assay g using a Gα11-Gβγ dissociation assay and h using Gαo-Gβγ dissociation assay. Data from three independent experiments (n = 3) are presented as mean ± SEM.

Back to article page