Fig. 3: Somatic hypermutation and germline features play a role in BG24 recognition of the CD4bs interface.
From: HIV-1 CD4-binding site germline antibody–Env structures inform vaccine design
gp120 (light gray) surface in the vicinity of the CD4bs with cartoon representation main chain/stick side chains for the CDRL1s of a BG24mat (light teal), b BG24iGL-CDR3mat (light purple), and c BG24iGL-CDR3iGL (light pink) overlaid with the N276gp120 N-glycan (dark blue) from the BG24mat-BG505 complex (PDB 7UCF). Steric clashes are represented with red bursts. d Table summarizing HC paratope residues in BG24iGL-CDR3iGL-GT1, BG24iGL-CDR3mat-GT1, and BG24mat-BG505 structures. The paratope was defined by Ab residues that make contacts with gp120 within 4 Å for each structure. Stick representations of the CDRH2 residues from e BG24mat (deep teal), f BG24iGL-CDR3mat (dark purple), and g BG24iGL-CDR3iGL (bright pink) interacting with BG505 or GT1 gp120 residues. Yellow dashed lines indicate Ab-gp120 interactions within 4 Å. h Neutralization data of BG24 CDRH2 mutants against a global 12 virus panel35 and BG505 T332N33. IC50 values represent the average mean from duplicate neutralization measurements. A heatmap describes <2-fold (white), 2–5-fold (light red), and >5-fold (dark red) potency decreases compared to BG24mat.
