Fig. 4: BG24iGL binding is mediated by CD4bs glycans.
From: HIV-1 CD4-binding site germline antibody–Env structures inform vaccine design
a ELISA to access binding of the indicated BG24 Abs to BG505, GT1, and GT1 SOSIP Envs with altered N-glycans in the CD4bs. Streptavidin plates were coated with randomly biotinylated SOSIPs and incubated with IgGs at increasing concentrations. Values are shown as mean of two individual biological replicates (n = 2). Source data are provided as a Source Data file. b Side and top-down views of cryo-EM density of BG24LC-iGL-GT1N276gp120-10-1074. Highlighted in colors include: gp120 subunits (light gray), gp41 (dark gray), BG24mat VH (deep coral), and VL (light coral) domains, and 10-1074 VH (dark brown) and VL (light brown) domains. c Cartoon representation of the CDLR1 of BG24LC-iGL (left) (light coral) and overlaid with the N276gp120 N-glycan (dark blue) from a BG24mat-BG505 (PDB 7UCF) (right). Predicted steric clashes are indicated by red bursts. d Alignment of BG24LC-iGL (from the BG24LC-iGL-GT1N276gp120−10-1074 structure) (light coral), IOMA (PDB 5T3Z) (lilac), and CLK31 (PDB 6D2P) (wheat) LC with CDRL1s represented in cartoon.
