Fig. 5: Non-engineered 6405 SOSIP recognizes BG24CDRL1-iGL.
From: HIV-1 CD4-binding site germline antibody–Env structures inform vaccine design
a Summary of neutralization of 6405 and 6952 pseudoviruses by BG24CDRL1-iGL IgGs. b ELISA to access binding of BG24-derived Abs to 6405 SOSIP. Streptavidin plates were coated with randomly biotinylated SOSIPs and incubated with BG24-derived IgGs, at increasing concentrations. Values are shown as mean of two individual biological replicates (n = 2) with associated. c Side and top-down views of cryo-EM density of BG24CDRL1-iGL−6405-10-1074. Highlighted in colors include: gp120 subunits (light gray), gp41 (dark gray), BG24CDRL1-iGL VH (dark green), and VL (light green) domains, and 10-1074 VH (dark brown) and VL (light brown) domains. d Surface contacts made by BG24CDRL1-iGL VH (dark green) and VL (light green) on 6405 gp120 (light gray). e Cartoon representation for the CDRL1 of BG24CDRL1-iGL (light green). f Alignment of GT1N276gp120 (light gray) and 6405 gp120s (light gray) in surface representation and N276 glycans (dark blue and deep teal) in sphere representation. g, h Summary for area under the curve (AUC) values derived from ELISAs that accessed binding of CD4bs IgGs to g 6405 and h 6405delN276gp120 SOSIPs. Streptavidin plates were coated with randomly biotinylated SOSIPs and incubated with CD4bs IgGs at increasing concentrations. Values are shown as mean of two individual biological replicates (n = 2). Source data are provided as a Source Data file.
