Fig. 2: HSF1 is dispensable for stressed hematopoiesis.

a Kaplan–Meier survival curves of Hsf1^fl/fl (Control, Con) or Hsf1^fl/flVav-Cre⁺ (Hsf1KO) mice treated with weekly doses of 5-FU (150 mg/kg, IP, Con n = 7 mice; Hsf1KO, n = 6 mice). b HSC (defined as Lin⁻cKit⁺Sca1⁺CD150⁺ CD48⁻) numbers of Con or Hsf1KO mice at different time points after sublethal (6.5 Gy) radiation (Con, n = 3 mice at each time points, Hsf1KO, n = 3 mice at Day 0 and 10, n = 5 mice at Day 30 and n = 4 at Day 45). c Percentages of HSCs in Con or Hsf1KO mice (3 mice/group) treated with 35 μg of LPS twice (48 h apart) and analyzed 24 h after the second injection. d Donor chimerisms of mice serially transplanted with control (Con) or Hsf1KO HSCs (primary, 500 cells/mouse, secondary and tertiary BMT, 2 × 10⁶ BM cells from primary or secondary recipients. e Frequencies of CD3⁺ T cells, B220⁺ B cells, Mac1⁺Gr1⁻ monocytes and Mac1⁺Gr1⁺ granulocytes in primary, secondary, and tertiary recipient mice transplanted with Con or Hsf1KO KLS cells. d, e n = 4/group for primary and secondary BMT, and n = 5/group for tertiary BMT. In (b–e), data are presented as mean values ± SEM. Source data are provided as a Source Data file.