Fig. 2: Kinetics of the c-JUN N-terminal phosphorylation in vivo. | Nature Communications

Fig. 2: Kinetics of the c-JUN N-terminal phosphorylation in vivo.

From: An intrinsic temporal order of c-JUN N-terminal phosphorylation regulates its activity by orchestrating co-factor recruitment

Fig. 2

a Representative Western blot analysis of the phosphorylation (activation) of endogenous JNK in NIH3T3 cells following anisomycin treatment (top), and quantification of the detected protein levels using the Image Studio Lite Software (Licor) and normalized to total JNK. Graphs show the mean ± standard error of the mean (SEM) (n = 3 biologically independent experiments) (bottom). b Representative western blot analysis of the phosphorylation kinetics of endogenous c-JUN in NIH3T3 cells following anisomycin treatment using phosphorylation-specific antibodies (top), and quantification of the detected protein levels using the Image Studio Lite Software (Licor) and normalized to total c-JUN and subsequently to the maximum phosphorylation time point of each phosphorylation-specific antibody. Graphs show the mean ± standard error of the mean (SEM) (n = 3 biologically independent experiments) (bottom). Source data are provided as a Source Data file. c Schematic summary of the observed phosphorylation kinetics of the c-JUN N-terminal phosphorylation by JNK.

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