Fig. 5: The purified Δ1–854 hTRPA1 displays various single-channel conductance states. | Nature Communications

Fig. 5: The purified Δ1–854 hTRPA1 displays various single-channel conductance states.

From: The human TRPA1 intrinsic cold and heat sensitivity involves separate channel structures beyond the N-ARD domain

Fig. 5

As shown by representative traces and corresponding histograms, several conductance states of which the main state had a conductance of 44–60 pS were identified at the temperatures investigated in the present study. Sub-conductance states were in the range of 14–43 pS, as also evident in Fig. 3a and Supplementary Fig. 3. The purified Δ1–854 hTRPA1 was reconstituted into planar lipid bilayers, and channel currents were recorded with the patch-clamp technique in a symmetrical K+ solution at a holding potential of +60 mV (upward deflection shows open-channel state).

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