Fig. 1: CDTb purification. | Nature Communications

Fig. 1: CDTb purification.

From: Cryo-EM structures of the translocational binary toxin complex CDTa-bound CDTb-pore from Clostridioides difficile

Fig. 1

a Protocol diagram. b Density gradient without LMNG pretreatment. c Density gradient with LMNG pretreatment before chymotrypsin treatment. d Location of CDTb residue F774 involved in di-heptamer formation. e Density gradient for the F774L mutant. f Density gradient for the F774G mutant. Both F774L and F774G were purified without LMNG pretreatment.

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