Fig. 4: Preparation and characterization of HM-SiO2 NPs.
From: Systematic design of cell membrane coating to improve tumor targeting of nanoparticles
a Schematic showing the process of developing fully coated HM-SiO2 NPs. b Cryo-TEM image of HM vesicles. Scale bar, 100 nm. c Size distribution of HM vesicles. d DSC analysis of HM vesicles prepared with CM protein/DOPC lipid weight ratios of 0, 0.04, 0.16, and 0.32. Typical peaks are indicated by black arrows. e, f Effects of CM incorporation on the laurdan GP (e) and fluorescence anisotropy (f) values of HM vesicles prepared at different weight ratios (protein/lipid). g Quantification of the ratio of full CM coating for SiO2 NPs coated with HM vesicles of different weight ratios (protein/lipid) and the plain CM coating with the same amount of CM protein (0.4 mg/mL) used in the HM coating. h Mean diameters and zeta potentials of SiO2 NPs, HM vesicles, and HM-SiO2 NPs. i TEM images of HM-SiO2 NPs fabricated by incubation, sonication, and extrusion. Scale bars, 100 nm. j Quantification of the ratio of full CM coating with different coating methods (incubation, sonication, and extrusion). k CLSM images showing the intracellular co-localization of lipid (labeled with NBD; green), CM materials (labeled with DiI; orange) and SiO2 NPs (labeled with Cy5; red) after internalization by CT26 cells. HM-SiO2 NPs were incubated with CT26 cells for 4 h. Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bar, 10 μm. l TEM images of HM vesicles and HM-SiO2 NPs stained with colloidal gold NPs (yellow arrows, ~5 nm). Scale bars, 25 nm. Experiments in panels b–d, i, k, and l were repeated three times independently with similar results. Data represent the mean ± s.d. (n = 3 independent experiments) in panels e−h and j. Significance was determined by one-way ANOVA followed by post hoc Tukey test in panels g and j. ****p < 0.0001. Source data are provided as a Source Data file.
